The technique involves several steps: 1. Biotinylation: The target molecule (e.g., protein or nucleic acid) is conjugated with biotin. 2. Binding: The biotinylated molecule is then introduced to streptavidin, which binds tightly to the biotin. 3. Detection: Streptavidin is often conjugated to a detectable label, such as an enzyme, fluorophore, or gold particle, allowing for visualization or quantification of the target molecule.
