Preparation of tissue samples involves several steps:
Fixation: Use formalin or another fixative to preserve tissue morphology. Dehydration: Gradually replace water in tissues with alcohol. Clearing: Replace alcohol with a substance like xylene that is miscible with embedding medium. Embedding: Immerse tissue in paraffin wax to solidify it for sectioning. Sectioning: Use a microtome to cut thin tissue sections. Staining: Apply stains such as Hematoxylin and Eosin (H&E) to highlight different tissue components.
