Trichrome stains utilize a sequential staining process where different dyes are applied to the tissue, each binding to specific tissue components. Typically, the staining protocol includes a fixative step, an initial stain for collagen (such as aniline blue or light green), a counterstain for muscle fibers (usually red), and a final stain for cell nuclei (commonly black or dark blue). The result is a multi-colored tissue section where collagen, muscle, and nuclei are distinctly colored.
