These tools utilize a variety of algorithms to analyze digital images of histological slides. The process typically involves several steps:
Image Acquisition: High-resolution images of tissue samples are captured using digital slides scanners. Preprocessing: The images are processed to enhance quality, such as adjusting contrast and removing artifacts. Segmentation: The software identifies and isolates different structures within the tissue, such as cells, nuclei, and extracellular matrix. Feature Extraction: Relevant features such as shape, size, and texture are extracted from the segmented structures. Classification and Quantification: The extracted features are used to classify and quantify specific histological characteristics, aiding in diagnosis.