Each technique has its own advantages and limitations:
- TEM: Provides the highest resolution and is ideal for viewing internal structures of cells. However, it requires extensive sample preparation and cannot be used on living cells. - SEM: Excellent for surface details and 3D reconstructions but offers less internal detail compared to TEM. - AFM: Offers high-resolution topographical imaging in three dimensions and can be used on living cells, though it has a slower scanning speed. - Super-Resolution Fluorescence Microscopy: Allows for imaging of living cells with specific molecular labeling, but typically requires fluorescent dyes and has a limited penetration depth.
