Pathologists use a variety of techniques to assess tumor aggressiveness. These include: - Histological Staining: Techniques like Hematoxylin and Eosin (H&E) staining help in visualizing cellular and tissue architecture. - Immunohistochemistry: This technique uses antibodies to detect specific proteins that may be associated with aggressive behavior. - Molecular Pathology: Genetic and molecular analyses can identify mutations and other alterations that drive aggressive tumor behavior.