How Do Minimal Light Conditions Interact with Different Staining Techniques?
Different staining techniques may require specific lighting: - Hematoxylin and Eosin (H&E): Typically observed in brightfield microscopy, but minimal light can still enhance contrast. - Fluorescent Staining: Minimal light conditions are crucial to prevent photobleaching and to see fluorescence clearly. - Immunohistochemistry: Requires careful light management to visualize antibody-antigen interactions.
