Fixation: Preserves the tissue by preventing decay and maintaining structure. Common fixatives include formalin and glutaraldehyde. Embedding: The tissue is embedded in a solid medium, usually paraffin wax, to allow for thin sectioning. Sectioning: Thin slices of the tissue are cut using a microtome. These sections are typically 4-10 micrometers thick. Staining: The sections are stained to highlight different cellular components and structures. Mounting: The stained sections are mounted on glass slides for examination under a microscope.
