How Do Histological Techniques Help in Studying Viral Morphology?
Several histological techniques are employed to study viral morphology:
Electron Microscopy: This technique provides high-resolution images of viral particles, allowing detailed observation of their structure. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) are commonly used. Immunohistochemistry: This technique uses antibodies to detect specific viral proteins in tissue samples. It helps in identifying the presence and distribution of viruses within cells and tissues. In Situ Hybridization: This technique uses labeled nucleic acid probes to detect viral genetic material in tissue sections. It is valuable for localizing viral genomes within infected cells. Light Microscopy: Although less detailed than electron microscopy, light microscopy can still reveal cytopathic effects (CPEs) of viral infections, such as cell enlargement, inclusion bodies, and syncytia formation.
