Cryogenic freezers utilize liquid nitrogen or mechanically driven refrigeration systems to achieve and maintain extremely low temperatures. Hereâs a brief overview of their operation: - Liquid Nitrogen Freezers: These freezers use liquid nitrogen, which boils at -196°C. Samples are stored in vials or cryo-tubes within the liquid nitrogen or its vapor phase. - Mechanical Cryogenic Freezers: These units employ advanced refrigeration cycles using gases such as helium or other cryogens to achieve temperatures around -150°C to -180°C.