Several methods can be employed to reduce autofluorescence:
1. Chemical Treatment: Use chemical quenching agents to reduce autofluorescence. 2. Spectral Unmixing: Apply spectral unmixing techniques to separate autofluorescence from specific signals. 3. Advanced Imaging: Utilize advanced imaging technologies like confocal microscopy or two-photon microscopy that can differentiate between autofluorescence and specific fluorophore emissions.
