How are Tissue Samples Prepared for Microscope Slides?
The preparation of tissue samples for microscope slides involves several steps:
Fixation: This process preserves the tissue's structure by using chemicals such as formaldehyde to prevent decay. Embedding: The fixed tissue is embedded in a solid medium, usually paraffin wax, to provide support for thin sectioning. Sectioning: A microtome is used to cut thin sections of the embedded tissue, typically 4-5 micrometers thick, which are then placed on the microscope slide. Staining: Stains such as hematoxylin and eosin (H&E) are applied to the sections to enhance contrast and highlight different structures within the tissue.
