The preparation and application of special stainers involve several steps:
Fixation: The tissue sample is fixed to preserve its structure and prevent degradation. Embedding: The fixed tissue is embedded in a medium like paraffin to facilitate sectioning. Sectioning: Thin sections of the tissue are cut using a microtome. Staining: The tissue sections are stained using the appropriate special stain protocol. Mounting: The stained sections are mounted on slides for microscopic examination.
