Several techniques are employed to detect SSBs in histological samples. The most common methods include:
1. Comet Assay: Also known as single cell gel electrophoresis, this technique allows the visualization of DNA damage at the level of individual cells. 2. Immunofluorescence: This method uses antibodies specific to DNA damage markers, such as poly (ADP-ribose) polymerase (PARP) or γ-H2AX. 3. TUNEL Assay: Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) detects DNA fragmentation by labeling the terminal end of nucleic acids.
