The preparation of histological images involves several steps. First, the tissue is fixed using chemicals like formaldehyde to preserve its structure. Then, the tissue is embedded in a medium such as paraffin wax to provide support. Thin slices of the tissue, known as sections, are cut and mounted on glass slides. These sections are then stained using various staining techniques to highlight different cellular components and structures. Common stains include hematoxylin and eosin (H&E), which color nuclei blue and cytoplasm pink, respectively.
