The procedure for enzyme activity assays typically involves several steps:
1. Sample Preparation: Tissues are homogenized, and the enzyme of interest is extracted. 2. Reaction Setup: The enzyme extract is mixed with a specific substrate under controlled conditions (pH, temperature). 3. Incubation: The reaction mixture is incubated for a defined period to allow the enzyme to act on the substrate. 4. Detection: The reaction product is measured using appropriate detection methods (colorimetric, fluorometric, spectrophotometric, or radiometric). 5. Data Analysis: The enzyme activity is calculated based on the amount of product formed, often expressed as units per milligram of protein.
