Controlled techniques in histology are crucial for ensuring consistency and reproducibility. This includes the use of standard protocols for fixation, embedding, sectioning, and staining of tissues. Each step must be performed under controlled conditions to maintain the integrity of the tissue samples. For example, during fixation, tissues are often treated with formalin or other fixatives to preserve cellular structures. The concentration, temperature, and duration of fixation are meticulously controlled to prevent over-fixation or under-fixation.
