Despite its benefits, there are some limitations and concerns associated with BrdU use:
Toxicity: BrdU can be toxic to cells at high concentrations, potentially affecting cell viability and proliferation rates. DNA Damage: Incorporation of BrdU can sometimes cause DNA strand breaks or mutations, which may interfere with normal cellular processes. Detection Complexity: The detection of BrdU-labeled cells requires denaturation of DNA, which can complicate the immunodetection process.
