What are histology storage containers?
In the field of
histology, storage containers are specially designed vessels used to store, transport, and preserve tissue samples. These containers are crucial for maintaining the integrity of samples from the time they are collected until they are analyzed under a microscope.
Types of storage containers
There are various types of storage containers used in histology, each serving a specific purpose: Specimen jars: Typically made of glass or plastic, these jars are used to store larger tissue samples. They often come with secure lids to prevent contamination.
Cryovials: These are small, usually plastic, containers used to store samples at very low temperatures, often in liquid nitrogen.
Paraffin blocks: After tissue is processed and embedded in paraffin wax, it is stored in these blocks for sectioning and further analysis.
Microscope slide holders: These containers are used to store and transport prepared slides, protecting them from damage and contamination.
Material consideration
The material of the storage container can significantly impact the preservation of the sample: Glass: Offers a non-reactive and transparent option, allowing for easy visual inspection of the sample. However, it is breakable.
Plastic: Lightweight and shatterproof, plastic containers are often used for their durability and flexibility.
Polypropylene: Commonly used for cryovials due to its ability to withstand extremely low temperatures without becoming brittle.
Sample type: Different tissues may require different containers. For example, fixed tissue samples might be stored in formalin-filled jars, while frozen samples might be kept in cryovials.
Storage conditions: Consider whether the sample needs to be stored at room temperature, refrigerated, or frozen.
Volume of sample: Ensure the container size is appropriate for the sample volume to avoid degradation.
Compatibility with fixatives: Some containers may react with certain chemicals, so compatibility is crucial.
Maintenance and labeling
Proper maintenance and labeling of storage containers are essential for the integrity of the samples: Cleaning: Containers must be thoroughly cleaned and sterilized before reuse to prevent cross-contamination.
Labeling: Accurate labeling with details such as sample ID, date of collection, and type of fixative used is critical for tracking and analysis.
Regulatory considerations
Histology storage containers must comply with certain
regulatory standards to ensure safety and efficacy. These standards are often set by governing bodies such as the FDA or ISO. Compliance ensures that the containers are safe for both the samples and the personnel handling them.
Conclusion
Storage containers in histology play a vital role in the preservation and integrity of tissue samples. Understanding the various types, materials, and best practices for choosing and maintaining these containers is essential for accurate and reliable histological analysis.
