Histology, the study of tissues at the microscopic level, involves various techniques and tools to ensure accurate analysis and documentation. One of the tools that has found its place in modern histological practices is the
infrared thermometer. Though primarily designed for measuring surface temperatures, infrared thermometers have diverse applications in histology labs.
How Do Infrared Thermometers Work?
Infrared thermometers function by detecting the infrared radiation emitted by an object. Every object with a temperature above absolute zero emits some form of infrared radiation, and the thermometer measures this radiation to determine the object's temperature without direct contact. This feature is particularly valuable in a
laboratory setting, where maintaining sterility and avoiding contamination are crucial.
Applications in Histology
In histology, maintaining the correct temperature is essential for various processes, including tissue fixation, staining, and embedding. Infrared thermometers offer a quick and non-invasive way to monitor temperatures, ensuring that all protocols are adhered to precisely. For instance, during tissue
fixation, the temperature of fixatives can be monitored to ensure optimal preservation of tissue morphology. Similarly, in
embedding procedures, the temperature of paraffin wax can be checked to prevent any damage to the tissue samples.
Benefits of Using Infrared Thermometers
The use of infrared thermometers in histology laboratories provides several advantages. Firstly, they offer a
non-contact method of temperature measurement, which is crucial for maintaining the integrity of the samples. Secondly, they provide immediate readings, allowing for real-time adjustments during experimental procedures. Additionally, these devices are versatile and can measure the temperature of a range of substances, from solid blocks of paraffin to liquid fixatives.
Limitations and Considerations
Despite their benefits, there are limitations to the use of infrared thermometers. The accuracy of the readings can be affected by the
emissivity of the object being measured. Different materials emit infrared radiation differently, so it is important to calibrate the thermometer according to the specific material being assessed. Moreover, infrared thermometers typically measure surface temperature, which might not always accurately reflect the internal temperature of a substance, such as a large volume of liquid.
Choosing the Right Infrared Thermometer
When selecting an infrared thermometer for histology applications, several factors should be considered. The
temperature range and accuracy are critical, as different histological processes may require precise temperature settings. The thermometer's durability, battery life, and ease of use are also important features to consider. Additionally, some advanced models come with features like adjustable emissivity settings and memory storage, which can be beneficial for specific laboratory needs.
Conclusion
Infrared thermometers have become an invaluable tool in histology laboratories, providing quick and accurate temperature measurements essential for various tissue processing techniques. By understanding the principles of their operation and carefully considering their applications and limitations, histologists can effectively integrate these devices into their workflows, enhancing the reliability and efficiency of their results.
