What is Histology?
Histology is the study of the microscopic anatomy of cells and tissues of plants and animals. It is performed by examining a thin slice (section) of tissue under a microscope. Histologists use various pieces of
equipment to prepare and examine these specimens.
Microtomes
A
microtome is used to cut extremely thin slices of tissue. These slices are then placed on glass slides for examination under a microscope. There are different types of microtomes including rotary, sliding, and cryostat microtomes.
Tissue Processors
A
tissue processor is an automated machine that processes tissue samples by fixing, dehydrating, clearing, and infiltrating them with wax. This prepares the tissues for slicing by a microtome.
Embedding Centers
An
embedding center is used to embed tissue samples in a medium, usually paraffin wax, to provide support during sectioning. This process ensures that the tissue retains its structure and is easier to cut.
Glass Slides and Coverslips
Glass slides are used to hold the thin tissue sections for microscopic examination. Coverslips are placed over the tissue on the slide to protect it and provide a clear view under the microscope.Digital Pathology Systems
With advances in technology,
digital pathology systems have become increasingly important. These systems scan and digitize tissue slides, allowing for easier sharing, analysis, and long-term storage of histological data.
Safety and Handling Equipment
Working with histological samples often involves hazardous chemicals. Safety equipment such as
fume hoods,
gloves, and
protective eyewear are essential for ensuring the safety of histologists.
Conclusion
The various pieces of equipment used in histology are integral to the accurate preparation, examination, and analysis of tissue samples. From microscopes to digital pathology systems, each tool plays a vital role in the field of histology.
