ACTB - Histology

What is ACTB?

ACTB, also known as Beta-Actin, is one of the six actin isoforms which are highly conserved proteins involved in various types of cell motility, structure, and integrity. In the context of Histology, it plays a crucial role in the formation of the cytoskeleton of eukaryotic cells.

Why is ACTB important in Histology?

ACTB is essential for maintaining the cell's shape, enabling cellular movements, and facilitating intracellular transport. It is often used as a housekeeping protein and a control marker for various immunohistochemical and molecular biology assays due to its consistent expression levels across different cell types.

How is ACTB detected in tissues?

ACTB can be detected using various immunohistochemistry (IHC) techniques. Antibodies specific to Beta-Actin are utilized to bind to the protein, allowing visualization under a microscope. Additionally, techniques such as Western blotting and RT-PCR are commonly employed to measure and confirm ACTB expression.

Applications of ACTB in Histological Studies

ACTB serves as a reliable internal control in gene expression studies and protein quantification. It assists researchers in normalizing data for comparative analysis. Its stable expression makes it invaluable in studies involving cell culture, tissue biopsies, and clinical diagnostics.

Challenges in Using ACTB as a Marker

Although ACTB is widely used as a control marker, it is not without its limitations. Variations in ACTB expression can occur in certain disease states or under specific experimental conditions, potentially leading to inaccurate data normalization. Hence, it is crucial to validate its expression stability in the context of the study being conducted.

Future Perspectives

The study of ACTB continues to evolve with advancements in molecular techniques and high-resolution imaging. Future research may uncover more precise roles of ACTB in various cellular processes and its potential as a therapeutic target in diseases involving cytoskeletal abnormalities.

Relevant Publications

Identifying the immunoglobulin G transporter in equine tissues: A look at the neonatal Fc receptor.

Issue Release: 2024

Elastin-derived peptides (EDPs) affect gene and protein expression in human mesenchymal stem cells (hMSCs) - preliminary study.

Issue Release: 2024

Integrated multi-omics and artificial intelligence to explore new neutrophils clusters and potential biomarkers in sepsis with experimental validation.

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Imaging the dynamics of messenger RNA with a bright and stable green fluorescent RNA.

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Physiological roles of propolis and red ginseng nanoplatforms in alleviating dexamethasone-induced male reproductive challenges in a rat model.

Issue Release: 2024

Mechanisms Involved in Therapeutic Effects of Georgi in Oral Squamous Cell Carcinoma Based on Systems Biology and Structural Bioinformatics Approaches.

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Selection of reference genes in liproxstatin-1-treated K562 Leukemia cells via RT-qPCR and RNA sequencing.

Issue Release: 2024

The Ross Procedure in a Case of Baraitser-Winter Syndrome: A Case Report.

Issue Release: 2024

Evaluation of reference genes for gene expression analysis by real-time quantitative PCR (qPCR) in different tissues from mice infected by Ascaris suum.

Issue Release: 2024

Molecular network mechanism in cerebral ischemia-reperfusion rats treated with human urine stem cells.

Issue Release: 2024

Ser252Trp mutation in fibroblast growth factor receptor 2 promotes branching morphogenesis in mouse salivary glands.

Issue Release: 2024

CRISPR/Cas9-mediated editing of ACTB in induced pluripotent stem cells: A model for investigating human ACTB loss-of-function and genetic adaptive responses.

Issue Release: 2024

Identification and functional characterization of annexin A2 in half-smooth tongue sole (Cynoglossus semilaevis).

Issue Release: 2024

A novel MTORC2-AKT-ROS axis triggers mitofission and mitophagy-associated execution of colorectal cancer cells upon drug-induced activation of mutant KRAS.

Issue Release: 2024

Prime-Editing of human ACTB in induced pluripotent stem cells to model human ACTB Loss-of-Function diseases and compensatory mechanisms.

Issue Release: 2024

Comparative transcriptome analysis of longissimus dorsi muscle reveal potential genes affecting meat trait in Chinese indigenous Xiang pig.

Issue Release: 2024

Proteomic profiling of laser capture microdissection kidneys from diabetic nephropathy patients.

Issue Release: 2024

Antifreeze protein type I in the vitrification solution improves the cryopreservation of immature cat oocytes.

Issue Release: 2024

Discovery of Novel TULP4/ACTN4/EWSR1/ACTB::MYB and ESRRG::DNM3 Fusions Expands Molecular Landscape of Adenoid Cystic Carcinoma Beyond Fusions Between MYB/MYBL1 and NFIB Genes.

Issue Release: 2024

Gastroblastoma with a novel ACTB::GLI1 gene fusion in a 19-year-old male.

Issue Release: 2024

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