What are the Steps Involved in Perls' Prussian Blue Staining?
1. Fixation: The tissue sample is fixed using a suitable fixative, commonly formalin. 2. Deparaffinization and Hydration: Paraffin-embedded tissue sections are deparaffinized and hydrated through graded alcohols to water. 3. Staining: The tissue is incubated in a mixture of hydrochloric acid and potassium ferrocyanide. 4. Counterstaining: The sections are counterstained with nuclear fast red to provide a contrasting background. 5. Dehydration and Mounting: The stained sections are dehydrated, cleared, and mounted for microscopic examination.
