The Golgi staining procedure typically involves several key steps: 1. Fixation: Tissue samples are fixed using a solution of formaldehyde or another fixative to preserve their structure. 2. Impregnation: The fixed tissue is then impregnated with a potassium dichromate solution, followed by silver nitrate. This step leads to the formation of a black precipitate within the neurons. 3. Sectioning: The impregnated tissue is sectioned into thin slices, usually using a microtome, to allow for detailed examination under a microscope. 4. Mounting and Observation: The tissue sections are mounted on glass slides and examined under a microscope to observe the stained neurons.
