What is Stropping?
Stropping is a technique used in histology to maintain the sharpness of microtome blades, which are essential for slicing thin sections of tissue for microscopic examination. The process involves drawing the blade over a surface, such as a leather strop, to realign the edge and remove any nicks or burrs.
Why is Stropping Important in Histology?
The quality of tissue sections directly impacts the accuracy of histological studies. A dull blade can cause tearing, crushing, or uneven sections, compromising the integrity of the samples. Therefore, regular stropping is crucial to ensure that blades remain sharp and produce high-quality sections.
How is Stropping Performed?
Stropping involves the following steps:
1.
Preparation: Ensure that the strop is clean and free of debris. Some histologists apply a fine abrasive paste for better results.
2.
Technique: Hold the blade at a consistent angle and draw it across the strop in a sweeping motion, alternating sides to evenly hone the edge.
3.
Inspection: After stropping, examine the blade under a microscope to confirm that it is free of nicks and has a uniformly sharp edge.
Types of Strops Used in Histology
Several types of strops are commonly used:
- Leather Strops: Traditional and effective, these strops are often treated with a fine abrasive compound.
- Synthetic Strops: Made from materials like canvas or rubber, these strops are durable and can be used with or without abrasive compounds.
- Diamond Paste Strops: These strops are infused with diamond particles, providing an exceptionally fine honing surface.Frequency of Stropping
The frequency of stropping depends on the type of tissue being sectioned and the quality of the microtome blade. Soft tissues may require less frequent stropping, whereas hard or calcified tissues can dull blades more quickly. A general guideline is to strop the blade after every 20-30 sections, but this can vary based on experience and observation.Common Mistakes in Stropping
Several common mistakes can reduce the effectiveness of stropping:
- Incorrect Angle: Holding the blade at the wrong angle can fail to properly hone the edge or even damage the blade.
- Uneven Pressure: Applying uneven pressure can create an inconsistent edge and reduce the blade's effectiveness.
- Over-stropping: Excessive stropping can wear down the blade unnecessarily, shortening its lifespan.Benefits of Proper Stropping
Proper stropping offers numerous benefits:
- Enhanced Sharpness: Maintains a keen edge, ensuring clean and precise tissue sections.
- Extended Blade Life: Regular maintenance can prolong the useful life of microtome blades.
- Improved Sample Quality: High-quality sections lead to more accurate and reliable histological analyses.Conclusion
Stropping is an essential skill in histology, playing a critical role in maintaining the sharpness of microtome blades. By ensuring that blades are regularly and properly stropped, histologists can produce high-quality tissue sections, leading to better diagnostic and research outcomes. Understanding the types of strops, correct techniques, and common pitfalls can significantly enhance the effectiveness of this important practice.
