What is Proper Storage in Histology?
Proper storage in histology involves preserving and maintaining the quality of histological samples, reagents, and equipment to ensure accurate and reliable results. Effective storage practices prevent deterioration, contamination, and degradation of these materials.
Maintains the
integrity of samples.
Prevents contamination.
Extends the shelf-life of reagents.
Ensures accurate and reproducible results.
Reduces waste and cost.
Tissue Sections: Store at -20°C or -80°C if long-term storage is needed. Avoid repeated freeze-thaw cycles.
Paraffin-embedded Blocks: Keep at room temperature in a dry, dark place. Use airtight containers to prevent oxidation.
Stained Slides: Store in slide boxes or cabinets at room temperature. Avoid prolonged exposure to light to prevent fading.
Fixatives: Store at room temperature in tightly sealed containers. Examples include formalin and glutaraldehyde.
Stains: Keep in dark, cool places to prevent degradation. Certain stains may require refrigeration.
Antibodies: Store at 4°C for short-term use or -20°C for long-term storage. Avoid repeated freeze-thaw cycles.
Microtomes: Keep in a clean, dust-free environment. Regularly lubricate moving parts.
Cryostats: Store at recommended temperatures. Follow manufacturer guidelines for maintenance.
Microscopes: Cover when not in use to prevent dust accumulation. Store in a cool, dry place.
Temperature Fluctuations: Can cause sample degradation.
Humidity: Excess moisture can lead to mold growth and sample degradation.
Light Exposure: Prolonged exposure can fade stains and damage samples.
Contamination: Improper sealing and handling can introduce contaminants.
Use temperature-controlled storage units.
Maintain a dry environment with dehumidifiers.
Use opaque containers to limit light exposure.
Implement strict handling protocols to prevent contamination.
CLSI: Provides standards for laboratory practices, including storage conditions.
CAP: Offers guidelines for pathology labs, including storage and maintenance.
FDA: Regulates storage conditions for reagents and medical devices used in histology.
Conclusion
Proper storage in histology is essential for maintaining the integrity and reliability of samples, reagents, and equipment. By adhering to best practices and regulatory guidelines, histologists can ensure accurate and reproducible results, ultimately contributing to high-quality research and diagnostics.
