Introduction
Hematoxylin and eosin (H&E) staining is one of the most commonly used techniques in the field of
histology. This staining method is essential for distinguishing between different cell types and tissue structures. The H&E stain provides a clear contrast between various cellular components, making it invaluable for both diagnostic and research purposes.
What is Hematoxylin?
Hematoxylin is a natural dye extracted from the heartwood of the logwood tree. It is used to stain cell nuclei blue or purple. Hematoxylin itself is not a stain; it must be oxidized to hematein and combined with a mordant, usually aluminum ions, to form a complex that binds to nucleic acids in the nucleus.
What is Eosin?
Eosin is a synthetic dye that stains the cytoplasm and extracellular matrix pink or red. It is an acidic dye that binds to basic (or eosinophilic) components of the cell, such as proteins. Eosin is often used in combination with hematoxylin to provide a clear contrast between different tissue components.
Fixation: Tissue samples are fixed in formalin to preserve their structure.
Embedding: The fixed tissues are embedded in paraffin wax to allow thin sectioning.
Sectioning: Thin sections of the tissue are cut using a microtome.
Staining: The sections are first stained with hematoxylin, followed by eosin.
Mounting: The stained sections are mounted on slides for microscopic examination.
Diagnostic pathology: Identifying and classifying diseases, such as cancer.
Research: Studying the structure and function of tissues.
Education: Teaching students about tissue morphology.
Contrast: Provides excellent contrast between different tissue components.
Simplicity: The staining procedure is straightforward and cost-effective.
Versatility: Suitable for a wide range of tissues and applications.
Specificity: Lacks the specificity of other staining methods, such as immunohistochemistry.
Resolution: May not reveal fine cellular details as well as other techniques.
Variability: Results can vary depending on the staining protocol and technician skill.
Conclusion
Hematoxylin and eosin staining remains a cornerstone in the field of histology. Its ability to provide clear, contrasted images of tissue structures makes it indispensable for both
diagnostic and research purposes. While it has some limitations, the advantages of H&E staining ensure its continued use in laboratories around the world.
