How Does FIA Work?
The process begins with the preparation of tissue sections, which are then incubated with a
primary antibody specific to the target antigen. A
secondary antibody conjugated with a fluorescent dye is then added. Upon binding, the fluorescent dye emits light when exposed to a specific wavelength, which can be detected and visualized using a
fluorescence microscope.
Conclusion
Fluorescence Immunoassay (FIA) is a powerful tool in histology, offering high sensitivity and specificity for detecting target molecules. With its wide range of applications and potential for multiplexing, FIA continues to be an essential technique for advancing our understanding of cellular and tissue functions.
